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Gene overexpression REST Us
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Gene overexpression stable cell lines are one of the widely used tools in biology research, including protein engineering, recombinant antibodies development, drug discovery, and other basic researches. Creative Biogene provides stable expression of a gene of interest (GOI) by plasmid transfection or lentivirus transduction.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/products/gene-overexpression.html

Luciferase REST Us
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Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/search?t=1&q=luciferase

HSV packaging REST Us
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Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. There are two HSV species: Herpes Simplex Virus Type I (HSV-1) and Herpes Simplex Virus Type II (HSV-2), both of which can infect humans and cause diseases. HSV genome replicates by means of a rolling circle mechanism which forms head-to-tail concatamers.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/services/Herpes-Simplex-Virus-(HSV)-Service.html

AAV packaging REST Us
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Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro). Though a number of viruses have been developed, interest has centered on four types: retrovirus, lentivirus, adenovirus and adeno-associated virus (AAV).

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/Services/Custom-Viral-Service

CRE adenovirus REST Us
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Cre adenoviral particle expresses Cre under control of a CMV promoter. Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/Cre-adenoviral-particles-AD00299Z-1233708-84.html

Human Retinal Pigment Epithelial Cells REST Us
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Human Retinal Pigment Epithelial Cells (HRPEpiC) are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Cr...

Provider: www-creative-bioarray-com | Base URL: http://www.creative-bioarray.com/Human-Retinal-Pigment-Epithelial-Cells-CSC-C1530-item-39346.htm

Hepatoma Cells REST Us
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Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.

Provider: www-creative-bioarray-com | Base URL: https://www.creative-bioarray.com/products/hepatoma-cells-list-97.htm

Human Hepatocytes REST Us
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HH are isolated from human liver. HH are cryopreserved immediately after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HH are characterized by immunofluorescent method with antibodies to albumin, cytokeratin-18 and vimentin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH is not recommend for expanding or long term cultures since the cells do not proliferate in culture.

Provider: www-creative-bioarray-com | Base URL: https://www.creative-bioarray.com/Human-Hepatocytes-CSC-C1495-item-39332.htm

Hep-3b cells REST Us
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Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.

Provider: www-creative-bioarray-com | Base URL: https://www.creative-bioarray.com/HEP-3B-CSC-C0245-item-1092.htm

human brain microvascular endothelial cells REST Us
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Human Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from human brain tissue. Human Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails.

Provider: www-creative-bioarray-com | Base URL: https://www.creative-bioarray.com/Human-Brain-Microvascular-Endothelial-Cells-CSC-C1503-item-39320.htm

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