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sgRNA library REST Us
Categories:
PASSED
CRISPR library screening is a high-throughput screening method of important molecular targets. An efficient CRISPR screen starts with a well-designed sgRNA library targeting the genes or loci of interest. The sgRNA library presents in two forms, pool and array. Either pooled library or arrayed library conducted, it has good performance in screening. SgRNA are designed based on sequence and then synthesis followed by cloning to vector or transcribing into RNA for transfection in cell system. T...

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/crispr-cas9/crispr-library-screening.html

sgRNA validation REST Us
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PASSED
Since the first description of CRISPR system, it becomes the most acceptable gene-editing tool in the world. The key components of CRISPR system are Cas9 nucleases and guide RNA. In term of targeting different sites genome, sgRNA varies the sequence to each target. A high-quality sgRNA is the foundation of successful gene-editing process. When to select the target sites of Cas9, the only requirement is the presence of PAM. There are multiple type Cas9 nucleases which require different PAMs.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/crispr-cas9/sgrna-design-and-confirmation.html

Primary T cell REST Us
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PASSED
The success of genome editing in primary T cell robustly promotes the development of CAR-T immunotherapy. In CAR-T cells, CAR gene can be delivered to a specific site within the genome by CRISPR/Cas9. Using CRISPR/Cas9 system reduces the risk of gene insertion at incorrect or undesired location. Meanwhile, CRISPR/Cas9 technology is used to knock out endogenous T cell receptor (TCR) and HLA class I (HLA-I) for reducing unwanted graft-versus-host reactivity.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/crispr-cas9/service/gene-editing-in-primary-t-cells.html

Off-target effects REST Us
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PASSED
Cas9, which is a nuclease, can be recruited to the specific site by guide RNA and then cleaves DNA. Similarly to other nucleases, Cas9 can cleave off-target DNA targets in the genome at reduced frequencies. For application of Cas9, it is essential to consider ways to minimize the degree of off-target cleavage and to detect the presence of off-target cleavage.

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/crispr-cas9/crispr-off-target-effects-analysis.html

microbial genome editing REST Us
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PASSED
Homologous recombination has been broadly applied in genome editing of prokaryotes with high efficiency and accuracy. However, this way is limited in realizing larger-scale genome editing with large DNA fragments or numerous genes because DNA editing template construction is a relatively complicated procedure1. Derived from the immune system present in bacteria and archaea, CRISPR technology as an efficient genome-scale editing tool has revolutionized conventional genetic engineering methods ...

Provider: www-creative-biogene-com | Base URL: https://www.creative-biogene.com/crispr-cas9/microbial-genome-editing-service.html

The G6GFINDR System REST
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The G6GFINDR System is a query-based system that helps you find bioinformatics and artificial intelligence (AI) software. At a future date, the system will be expanded to include more specific recommendations and also to other subject areas. G6GFINDR is powered by "semantic annotation" vs. keyword search. Semantic annotations take advantage of semantic web graph technology. Semantic annotation is the process of creating meta-data (annotations) from unstructured data based on Natural L...

Provider: g6gfindr-com | Base URL: https://g6gfindr.com

Proteins API REST
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The Proteins REST API provides access to key biological data from UniProt and data from Large Scale Studies data mapped to UniProt. The services provide sequence feature annotations from UniProtKB, variation data from UniProtKB and mapped from Large Scale data sources (1000 Genomes, ExAC and COSMIC), proteomics data mapped from Large Scale sources (PeptideAtlas, MaxQB and EPD) and genome coordinate mappings.The interactive documentation provides dynamically generated source code for Java, P...

Provider: European Bioinformatics Institute (EMBL-EBI) | Base URL: http://www.ebi.ac.uk/proteins/api

Brassica Information Portal REST Gb
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The Brassica Information Portal is a web repository for population and trait scoring information related to the Brassica breeding community. It provides information about quantitative trait loci and links curated Brassica phenotype experimental data with genotype information stored in external data sources. Advanced data submission capabilities and APIs enable users to store and publish their own study results in our Portal. The repository can be easily browsed thanks to a set of user-friendl...

Provider: bip-earlham-ac-uk | Base URL: http://bip.earlham.ac.uk

Disorder Atlas REST Us
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Disorder Atlas is a web-based service that facilitates the interpretation of intrinsic disorder predictions using proteome-based descriptive statistics. This service is also equipped to facilitate large-scale systematic exploratory searches for proteins encompassing disorder features of interest, and further allows users to browse the prevalence of multiple disorder features at the proteome level. Disorder Atlas is freely available for non-commercial users at http://www.disorderatlas.org.

Provider: University of Michigan Medical School | Base URL: http://www.disorderatlas.org

Arabidopsis thaliana Gene annotation information (Araport) REST Us
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The Arabidopsis Information Portal is an open-access online community resource for Arabidopsis research. Araport enables biologists to navigate from the Arabidopsis thaliana Col-0 reference genome sequence to its associated annotation including gene structure, gene expression, protein function, and interaction networks. This service provides gene annotation information from ThaleMine (an Arabidopsis thaliana specific instance of InterMine) given an AGI locus identifier (e.g. AT1G65480).

Provider: api-araport-org | Base URL: https://api.araport.org/community/v0.3/araport/gene_summary_by_locus_v0.2

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