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Overview
Provider:
European Bioinformatics Institute (EMBL-EBI)
Location:
United Kingdom
Submitter/Source:
Hamish McWilliam
(almost 11 years ago)
Base URL:
http://www.ebi.ac.uk/Tools/services/soap/psiblast
WSDL Location:
http://www.ebi.ac.uk/Tools/services/soap/psiblast?wsdl (download last cached WSDL file)
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Position Specific Iterative BLAST (PSI-BLAST) refers to a feature of NCBI BLAST 2.0 in which a position-specific scoring matrix (PSSM) profile is constructed from set of BLAST alignments. The PSSM is then used to search the database in subsequent iterations allowing the detection of distant evolutionary relationships. This can be combined with the Pattern Hit Initiated BLAST (PHI-BLAST) mode to constrain the PSSM using a ProSite pattern to define the region(s) to be used when building the PSSM.
![header=[] body=[An ELIXIR Description must be a short (maximum of 2 sentences), succinct description of the Webservice. It should no contain links or be overly technical. If you are unsure, this should be left blank.] cssheader=[boxoverTooltipHeader] cssbody=[boxoverTooltipBody] delay=[200] Info](/assets/info-092c542cd75fb00e5270a8b7c81dc616.png)
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Position Specific Iterative BLAST (PSI-BLAST) refers to a feature of NCBI BLAST 2.0 in which a position-specific scoring matrix (PSSM) profile is constructed from set of BLAST alignments.
![header=[] body=[What are the usage conditions on this service? For example: not allowed more than 2 requests per minute and 100 requests per hour.] cssheader=[boxoverTooltipHeader] cssbody=[boxoverTooltipBody] delay=[200] Info](/assets/info-092c542cd75fb00e5270a8b7c81dc616.png)
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E-mail supplied for submission must be a valid e-mail address and no more than 25 concurrent jobs should be submitted at any one time, see http://www.ebi.ac.uk/Tools/webservices/about/webservices and the EMBL-EBI Terms of Use: http://www.ebi.ac.uk/Information/termsofuse.html
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![header=[] body=[What papers have been published <b>for</b> this service (this can be in a common citation format like Bibtex, MLA or APA, a DOI, a URL, etc.)] cssheader=[boxoverTooltipHeader] cssbody=[boxoverTooltipBody] delay=[200] Info](/assets/info-092c542cd75fb00e5270a8b7c81dc616.png)
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Goujon M., McWilliam H., Li W., Valentin F., Squizzato S., Paern J. and Lopez R. (2010) “A new bioinformatics analysis tools framework at EMBL-EBI.” Nucleic Acids Res. 38:W695-9 PMID: 20439314 (http://www.ebi.ac.uk/citexplore/citationDetails.do?externalId=20439314&dataSource=MED) DOI: 10.1093/nar/gkq313 (http://dx.doi.org/10.1093/nar/gkq313)
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Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W. and Lipman D.J. (1997) “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.” Nucleic Acids Res. 25(17):3389-402. PMID: 9254694 (http://www.ebi.ac.uk/citexplore/citationDetails.do?externalId=9254694&dataSource=MED) DOI: 10.1093/nar/25.17.3389 (http://dx.doi.org/10.1093/nar/25.17.3389)
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