Web Service sgRNA validation REST Us

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Bennie GeorgeUs (2 months ago)

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by Bennie George (2 months ago)

Since the first description of CRISPR system, it becomes the most acceptable gene-editing tool in the world. The key components of CRISPR system are Cas9 nucleases and guide RNA. In term of targeting different sites genome, sgRNA varies the sequence to each target. A high-quality sgRNA is the foundation of successful gene-editing process. When to select the target sites of Cas9, the only requirement is the presence of PAM. There are multiple type Cas9 nucleases which require different PAMs.

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