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Bennie GeorgeUs (2 months ago)

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by Bennie George (2 months ago)

CRISPR library screening is a high-throughput screening method of important molecular targets. An efficient CRISPR screen starts with a well-designed sgRNA library targeting the genes or loci of interest. The sgRNA library presents in two forms, pool and array. Either pooled library or arrayed library conducted, it has good performance in screening. SgRNA are designed based on sequence and then synthesis followed by cloning to vector or transcribing into RNA for transfection in cell system. The transfected cells are detected for pre-set parameters and the results are analyzed at final.

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