European Bioinformatics Institute (EBI)

United Kingdom

Submitter / Source:
Hamish McWilliam (about 1 year ago)

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Documentation URL(s): Hamish McWilliam (about 1 year ago)

Login to add a documentation URL Description(s): Hamish McWilliam (about 1 year ago)MUSCLE (MUltiple Sequence Comparison by Log-Expectation) is claimed to achieve both better average accuracy and better speed than ClustalW or T-Coffee, depending on the chosen options. Multiple alignments of protein sequences are important in many applications, including phylogenetic tree estimation, secondary structure prediction and critical residue identification.

Login to add a description License(s): Hamish McWilliam (11 months ago)EMBL-EBI Terms of Use:

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Login to add cost info Usage Conditions: Hamish McWilliam (11 months ago)E-mail supplied for submission must be a valid e-mail address and no more than 25 concurrent jobs should be submitted at any one time, see and the EMBL-EBI Terms of Use:

Login to add usage conditions info Contact Info: Hamish McWilliam (11 months ago)EMBL-EBI Support:

Login to add contact info Publications: for this service. This can be a URI to the publication and/or a DOI. Hamish McWilliam (3 days ago)Goujon M., McWilliam H., Li W., Valentin F., Squizzato S., Paern J. and Lopez R. (2010)
“A new bioinformatics analysis tools framework at EMBL-EBI.”
Nucleic Acids Res. 38:W695-9
PMID: 20439314 (;=MED)
DOI: 10.1093/nar/gkq313 (

Hamish McWilliam (3 days ago)Edgar R.C. (2004)
“MUSCLE: multiple sequence alignment with high accuracy and high throughput.”
Nucleic Acids Res. 32(5):1792-7.
PMID: 15034147 (;=MED)
DOI: 10.1093/nar/gkh340 (

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